Impact of long-term storage on the quality of frozen pickled sweet peppers

Keywords:

Sweet pepper, marinade, freezing, thawing, pickled vegetable, frozen vegetable, thawed vegetable, microwave-thawed vegetable, microflora, oxidative enzymes

Synopsis

Vegetables are produced seasonally; therefore, freezing is one of the main methods of preserving them while maintaining their quality. Both fresh vegetables and semi-finished or ready-to-eat vegetable products are commonly frozen. Such products are popular among consumers, as they provide rapid preparation and consumption. Vegetables, particularly sweet peppers, are often pickled in marinades with various spices to enhance sensory properties, preserve nutrients, extend shelf life, and expand the range of available products. A promising approach for storing pickled sweet peppers is freezing. In this study, sweet peppers were pickled in a marinade containing the following ingredients: water, sunflower oil, sugar, natural honey, salt, citric acid, and spices (dried dill and parsley, bay leaves, fresh garlic, and allspice). After washing with water, cleaning, and cutting, sweet pepper slices were pickled in the marinade for 12 h at room temperature and subsequently frozen at –20ºC. Experimental results showed that during 270 days of frozen storage of pickled sweet peppers, losses of ascorbic acid ranged from 12.0 to 19.8%, carotenoids from 5.8 to 15.1%, dry matter from 0.7 to 4.9%, total sugars from 0.6 to 6.1%. At the same time, the contents of water-soluble pectin, anthocyanins, catechins, and total flavonoids increased during storage. Freezing also had a positive effect on the microbiological safety of the product, as the number of microorganisms in the frozen product during storage was lower than that in fresh sweet peppers. The sensory quality of the frozen product, including appearance, color, aroma, taste, and consistency, was highly rated by expert evaluators. To bring the product quickly to a ready-to-eat state, microwave thawing is recommended. Pickled sweet peppers thawed using this technique exhibited high sensory quality. However, prolonged storage of the product at room temperature after thawing is not recommended, as it leads to a decrease in ascorbic acid content and an increase in polyphenol oxidase activity and microbial growth. Although the product remained safe for consumption after 24 h of storage at 20ºC, the number of microorganisms did not exceed the permissible limits for quick-frozen vegetables (the maximum bacterial and mold counts in the thawed pickled sweet peppers were 48,400 CFU/mm2 and 19.0 CFU/mm2, respectively).

References

Abdalla, M. U. E., Taher, M., Sanad, M. I., Tadros, L. K. (2019). Chemical Properties, Phenolic Profiles and Antioxidant Activities of Pepper Fruits. Journal of Agricultural Chemistry and Biotechnology, 10 (7), 133–140. https://doi.org/10.21608/jacb.2019.53475

Acheampong, R., Osei Tutu, C., Akonor, P. T., Asiedu, B. K., Mahama, S., Owusu-Bempah, J. et al. (2025). Effect of conventional and emerging thawing technologies on drip loss, microstructure and post-thaw quality of frozen fruits and vegetables: A review. Applied Food Research, 5 (2), 101323. https://doi.org/10.1016/j.afres.2025.101323

Ahmada Kh, A., A. A Abdo, A., Khan, S., Aleryani, H., Mi, S., Wang, X. (2025). Advancing Pickling Techniques to Enhance Bioactive Compounds and Probiotic Content in Pickled Vegetables. Food Reviews International, 42 (1), 31–57. https://doi.org/10.1080/87559129.2025.2473009

Alabi, K. P., Olalusi, A. P., Olaniyan, A. M., Fadeyibi, A., Gabriel, L. O. (2022). Effects of osmotic dehydration pretreatment on freezing characteristics and quality of frozen fruits and vegetables. Journal of Food Process Engineering, 45 (8). https://doi.org/10.1111/jfpe.14037

Barbagallo, R. N., Chisari, M., Patané, C. (2012). Polyphenol oxidase, total phenolics and ascorbic acid changes during storage of minimally processed ‘California Wonder’ and ‘Quadrato d’Asti’ sweet peppers. LWT – Food Science and Technology, 49 (2), 192–196. https://doi.org/10.1016/j.lwt.2012.06.023

Brezeanu, C., Brezeanu, P. M., Stoleru, V., Irimia, L. M., Lipșa, F. D., Teliban, G.-C. et al. (2022). Nutritional Value of New Sweet Pepper Genotypes Grown in Organic System. Agriculture, 12 (11), 1863. https://doi.org/10.3390/agriculture12111863

Cabrera, M., Muhammad, S., Rodriguez, E., Sommerhalter, M. (2024). Biochemical Laboratory Experiments on Polyphenol Oxidase. Journal of Chemical Education, 101 (8), 3500–3505. https://doi.org/10.1021/acs.jchemed.4c00533

Çalışkan Koç, G., Özkan Karabacak, A., Süfer, Ö., Adal, S., Çelebi, Y., Delikanlı Kıyak, B. et al. (2025). Thawing frozen foods: A comparative review of traditional and innovative methods. Comprehensive Reviews in Food Science and Food Safety, 24 (2). https://doi.org/10.1111/1541-4337.70136

Caruso, G., Stoleru, V. V., Munteanu, N. C., Sellitto, V. M., Teliban, G. C., Burducea, M. et al. (2018). Quality Performances of Sweet Pepper under Farming Management. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47 (2), 458–464. https://doi.org/10.15835/nbha47111351

Chen, B., Zhang, M., Wang, Y., Devahastin, S., Yu, D. (2022). Comparative study of conventional and novel combined modes of microwave - and infrared-assisted thawing on quality of frozen green pepper, carrot and cantaloupe. LWT, 154, 112842. https://doi.org/10.1016/j.lwt.2021.112842

Chen, B., Zhang, M., Wang, Y., Mujumdar, A. S., Yu, D., Luo, Z. (2023). Freezing of green peppers assisted by combined electromagnetic fields: Effects on juice loss, moisture distribution, and microstructure after thawing. Journal of Food Process Engineering, 46 (5). https://doi.org/10.1111/jfpe.14318

Cheng, J., Shen, H., Yang, X., Yu, S., Yuan, L., Sun, Z. et al. (2008). Changes in biochemical characteristics related to firmness during fruit development of pepper (Capsicum annuum L.). European Journal of Horticultural Science, 155–161. https://doi.org/10.1079/ejhs.2008/723249

Choskit, T., Gupta, N., Singh, J., Bhat, A., Bandral, J. D., Sood, M. et al. (2023). An overview on food spoilage mechanism and their prevention. Chemical Science Review and Letters, 12 (45), 60–66. Available at: https://chesci.com/wp-content/uploads/2023/04/v12i45_9_CS205312563_Completed-1.pdf

Cuadra‐Crespo, P., del Amor, F. M. (2010). Effects of postharvest treatments on fruit quality of sweet pepper at low temperature. Journal of the Science of Food and Agriculture, 90 (15), 2716–2722. https://doi.org/10.1002/jsfa.4147

Dudarev, I., Kuzmin, O., Stukalska, N., Antonenko, A., Brovenko, T., Kovalenko, N. et al. (2024). Using oat milk to reduce the caloric value of a functional mayonnaise sauce. Acta Scientiarum Polonorum Technologia Alimentaria, 23 (1), 29–38. https://doi.org/10.17306/j.afs.001184

Giannakourou, M. C., Dermesonlouoglou, E. K., Taoukis, P. S. (2020). Osmodehydrofreezing: An Integrated Process for Food Preservation during Frozen Storage. Foods, 9 (8), 1042. https://doi.org/10.3390/foods9081042

Giannakourou, M. C., Taoukis, P. S. (2021). Effect of Alternative Preservation Steps and Storage on Vitamin C Stability in Fruit and Vegetable Products: Critical Review and Kinetic Modelling Approaches. Foods, 10 (11), 2630. https://doi.org/10.3390/foods10112630

Grover, Y., Negi, P. S. (2023). Recent developments in freezing of fruits and vegetables: Striving for controlled ice nucleation and crystallization with enhanced freezing rates. Journal of Food Science, 88 (12), 4799–4826. https://doi.org/10.1111/1750-3841.16810

Güneş, R., Çetin, B. (2020). Investigation of some quality parameters of pickled pepper produced by low value dairy by-products. Gıda, 45 (3), 448–460. https://doi.org/10.15237/gida.gd19160

Hallmann, E., Marszałek, K., Lipowski, J., Jasińska, U., Kazimierczak, R., Średnicka-Tober, D. et al. (2019). Polyphenols and carotenoids in pickled bell pepper from organic and conventional production. Food Chemistry, 278, 254–260. https://doi.org/10.1016/j.foodchem.2018.11.052

Haron, H., Hassan, S., Chan, B. K. (2017). Evaluation of Total Phenolic Content, Antioxidant Activities and Sugar Content of Fresh Mixed Fruit and Vegetables Juices. Jurnal Sains Kesihatan Malaysia, 15 (2), 53–58. https://doi.org/10.17576/jskm-2017-1502-07

Janiszewska-Turak, E., Witrowa-Rajchert, D., Rybak, K., Rolof, J., Pobiega, K., Woźniak, Ł. et al. (2022). The Influence of Lactic Acid Fermentation on Selected Properties of Pickled Red, Yellow, and Green Bell Peppers. Molecules, 27 (23), 8637. https://doi.org/10.3390/molecules27238637

Kacmaz Ozcetin, S., Artok, L. (2025). Effect of Marination on the Formation of Polycyclic Aromatic Hydrocarbons in Grilled Vegetables. Food Science & Nutrition, 13(7). https://doi.org/10.1002/fsn3.70600

Kaur, M., Kumar, M. (2020). An Innovation in Magnetic Field Assisted Freezing of Perishable Fruits and Vegetables: A Review. Food Reviews International, 36 (8), 761–780. https://doi.org/10.1080/87559129.2019.1683746

Kowalska, B., Szczech, M. (2022). Differences in microbiological quality of leafy green vegetables. Annals of Agricultural and Environmental Medicine, 29 (2), 238–245. https://doi.org/10.26444/aaem/149963

Liu, D.-K., Xu, C.-C., Guo, C.-X., Zhang, X.-X. (2020). Sub-zero temperature preservation of fruits and vegetables: A review. Journal of Food Engineering, 275, 109881. https://doi.org/10.1016/j.jfoodeng.2019.109881

Neri, L., Faieta, M., Di Mattia, C., Sacchetti, G., Mastrocola, D., Pittia, P. (2020). Antioxidant Activity in Frozen Plant Foods: Effect of Cryoprotectants, Freezing Process and Frozen Storage. Foods, 9 (12), 1886. https://doi.org/10.3390/foods9121886

Pérez-López, A. J., López-Nicolas, J. M., Núñez-Delicado, E., Amor, F. M. del, Carbonell-Barrachina, Á. A. (2007). Effects of Agricultural Practices on Color, Carotenoids Composition, and Minerals Contents of Sweet Peppers, cv. Almuden. Journal of Agricultural and Food Chemistry, 55 (20), 8158–8164. https://doi.org/10.1021/jf071534n

Raffo, A., Baiamonte, I., Paoletti, F. (2007). Changes in antioxidants and taste-related compounds content during cold storage of fresh-cut red sweet peppers. European Food Research and Technology, 226 (5), 1167–1174. https://doi.org/10.1007/s00217-007-0646-4

Rao, T. V. R., Gol, N. B., Shah, K. K. (2011). Effect of postharvest treatments and storage temperatures on the quality and shelf life of sweet pepper (Capsicum annum L.). Scientia Horticulturae, 132, 18–26. https://doi.org/10.1016/j.scienta.2011.09.032

Rehman, R. N. U., Malik, A. U., Khan, A. S., Hasan, M. U., Anwar, R., Ali, S. et al. (2021). Combined application of hot water treatment and methyl salicylate mitigates chilling injury in sweet pepper (Capsicum annuum L.) fruits. Scientia Horticulturae, 283, 110113. https://doi.org/10.1016/j.scienta.2021.110113

Roy, M. K., Juneja, L. R., Isobe, S., Tsushida, T. (2009). Steam processed broccoli (Brassica oleracea) has higher antioxidant activity in chemical and cellular assay systems. Food Chemistry, 114 (1), 263–269. https://doi.org/10.1016/j.foodchem.2008.09.050

van der Sman, R. G. M. (2020). Impact of Processing Factors on Quality of Frozen Vegetables and Fruits. Food Engineering Reviews, 12 (4), 399–420. https://doi.org/10.1007/s12393-020-09216-1

Vardanian, I., Sargsyan, G., Martirosyan, G., Shirvanyan, A., Tadevosyan, L., Avagyan, A. et al. (2025). Comprehensive agrobiological and biochemical study of sweet pepper (Capsicum annuum L.) varieties. Functional Food Science, 5 (6), 205–222. https://doi.org/10.31989/ffs.v5i6.1640

Wang, J., He, J., Zhang, R., Li, N., Zhang, S., Li, J. et al. (2025). Comparative Study of Pigment Content, Nutrient Composition and Antioxidant Capacity of Different Color Peppers at Different Maturity Stages. Horticulturae, 11 (12), 1481. https://doi.org/10.3390/horticulturae11121481

Wang, Q., Ding, T., Zuo, J., Gao, L., Fan, L. (2016). Amelioration of postharvest chilling injury in sweet pepper by glycine betaine. Postharvest Biology and Technology, 112, 114–120. https://doi.org/10.1016/j.postharvbio.2015.07.008

Wu, J., Jia, X., Fan, K. (2022). Recent advances in the improvement of freezing time and physicochemical quality of frozen fruits and vegetables by ultrasound application. International Journal of Food Science & Technology, 57 (6), 3352–3360. Portico. https://doi.org/10.1111/ijfs.15744

Yu, H., Mei, J., Xie, J. (2022). New ultrasonic assisted technology of freezing, cooling and thawing in solid food processing: A review. Ultrasonics Sonochemistry, 90, 106185. https://doi.org/10.1016/j.ultsonch.2022.106185

Zahorko, N., Dudarev, I., Tkachuk, V.; Priss, O. (Ed.) (2025). Changes in quality parameters of sweet peppers during low-temperature storage after freezing. Innovative Approaches in Food Processing and Sustainability. Tallinn: Scientific Route OÜ. 195–217. https://doi.org/10.21303/978-9908-9706-2-2.ch10

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Zahorko, N., Dudarev, I., & Tkachuk, V. (2026). Impact of long-term storage on the quality of frozen pickled sweet peppers . In O. Priss, L. Bal-Prylypko, H. Tolok, S. Tolok, I. Ustymenko, I. Bal, O. Kanishchev, V. Davydovych, L. Shevchenko, O. Semenko, N. Slobodyanyuk, O. Pylypchuk, A. Ivaniuta, N. Holembovska, V. Israelian, A. Omelian, I. Ivanova, M. Serdyuk, T. Tymoshchuk, … L. Chepurda, Advances in food technology and innovation (pp. 220-241). Scientific Route OÜ®. https://doi.org/10.21303/978-9908-845-03-6.ch9