

Innovative approaches in food processing and sustainability
The growing interest in functional meat and fish-based foods has driven the development of novel formulations enriched with biologically active and nutritionally beneficial ingredients. This research explores the impact of incorporating unconventional components – such as chicken meat, spelt flour, dried vegetables, cuttlefish ink, and red caviar – into the composition of cooked fish sausages made primarily from hake. Three experimental formulations were created by substituting 10% of the fish meat with chicken and adding 6% spelt flour. Additionally, each variant included one of the following: bell pepper, olives, or garlic.
The study evaluated various parameters, including proximate composition (moisture, protein, fat, ash), water-holding and moisture-binding capacities, texture-related properties (shear stress), oxidative stability (acid and peroxide values), and sensory characteristics. Protein content in the modified products increased by 8–10% relative to the control (17.2 vs. 15.1 g/100 g). Among the samples, the first exhibited the highest water-holding capacity (86.3%), while the second and third demonstrated a notable 18–20% enhancement in structural density.
Over a 10-day refrigerated storage period, the third sample maintained superior oxidative stability, as its acid value increased only slightly (from 1.7 to 2.3 mg KOH/g fat). Sensory evaluation revealed improved acceptability: sample 1 achieved a score of 4.6, and sample 3 received 4.5, both outperforming the control 3.8.
Overall, the integration of chicken meat, spelt flour, and dried vegetable inclusions into fish sausage formulations resulted in improved nutritional quality, functional-technological performance, and organoleptic appeal. These findings support the potential use of alternative raw materials in the production of value-added, health-oriented fish sausages. Further research is recommended to assess microbial stability over extended storage periods and to determine consumer preferences for potential market introduction.
PhD, Associate Professor
Department of Meat, Fish and Seafood Technology
https://orcid.org/0000-0001-8159-4020
Corresponding author
natashagolembovska@gmail.com
PhD, Professor
Department of Meat, Fish and Seafood Technology
https://orcid.org/0000-0002-7724-2919
PhD, Associate Professor
Department of Meat, Fish and Seafood Technology
https://orcid.org/0000-0002-7242-3227
PhD Student
Department of Meat, Fish and Seafood Technology
https://orcid.org/0000-0003-4796-2445
PhD, Associate Professor
Department of Technologies in Poultry, Pig and Sheep Breeding
https://orcid.org/0000-0003-2286-0776
PhD, Associate Professor
Department of Technologies in Poultry, Pig and Sheep Breeding
https://orcid.org/0000-0002-6969-2987
PhD, Associate Professor
Department of Animal and Food Hygiene named after Professor A. K. Skorokhodko
https://orcid.org/0000-0003-0578-8856
PhD, Associate Professor
Department of Physiology, Plant Biochemistry and Bioenergetics
https://orcid.org/0000-0003-1939-2967
Israelian, V., Palamarchuk, I., Sevin, S., Holembovska, N., Prokopenko, N., Ivaniuta, A. et al. (2022). The effect of vibration massage on the salting process of ostrich meat. Potravinarstvo Slovak Journal of Food Sciences, 16, 530–544. https://doi.org/10.5219/1775
Iakubchak, O., Adamenko, L., Taran, T., Sydorenko, O., Rozbytska, T., Tverezovska, N. et al. (2023). The study of the cytotoxic effect of disinfectants. Potravinarstvo Slovak Journal of Food Sciences, 17, 82–95. https://doi.org/10.5219/1822
Stukalska, N. M., Niemirich, O. V., Hrytskevych, A. O. (2024). Study of the functional and technological properties of fish semi-finished products with the addition of hydrobiont processing products. Taurida Scientific Herald. Series: Technical Sciences, 2, 209–217. https://doi.org/10.32782/tnv-tech.2024.2.25
Prylipko, T. M., Kostash, V. B., Kuzminska, I. M. (2024). Improvement of the elements of the technology of manufacturing fish battle with the use of biologically active additives. Herald of Lviv University of Trade and Economics Technical Sciences, 37, 42–48. https://doi.org/10.32782/2522-1221-2024-37-06
Shurduk, I., Serik, M., Antonenko, S., Fedak, N. (2014). Effect of protein and mineral additive on consumer characteristics of meat emulsion products. Ukrainian Food Journal, 3 (4), 524–665.
Shtonda, O. A. (2004). Rozrobka tekhnolohii kovbasnykh vyrobiv z vykorystanniam horokhu. [Extended abstract of PhD thesis; National University of Food Technologies]. Available at: https://dspace.nuft.edu.ua/handle/123456789/7509
Zhao, X., Zhang, Z., Cui, Z., Manoli, T., Yan, H., Zhang, H. et al. (2022). Quality changes of sous-vide cooked and blue light sterilized Argentine squid (Illex argentinus). Potravinarstvo Slovak Journal of Food Sciences, 16, 175–186. https://doi.org/10.5219/1731
Bozhko, N. V., Tyshchenko, V. I. (2014). The use of antioxidants in technology of production of the boiled sausage. Bulletin of Sumy National Agrarian University. The series: Livestock, 2 (2), 154–159.
Geredchuk, A., Pasichnyi, V., Matsuk, Y., Kostenko, V. (2023). Development of technology of fish cutted semi-finished products with vegetable enrichers. Science Bulletin of Poltava University of Economics and Trade. Series "Technical Sciences", 2, 31–35. https://doi.org/10.37734/2518-7171-2022-2-5
Fursik, O. (2020). Improving the technology of coocked sausage products using composition containing protein. [Doctoral dissertation; National University of Food Technologies]. Available at: https://dspace.nuft.edu.ua/handle/123456789/31891
Martyniuk, I. O. (2008). Particularity of microstructure of boiled sausages with amaranth flour. Scientific Bulletin of the S. Z. Gzhytsky Lviv National University of Veterinary Medicine and Biotechnology, 10 (2-5 (37)), 86–89. Available at: https://cyberleninka.ru/article/n/osoblivosti-mikrostrukturi-varenih-kovbasnih-virobiv-z-amarantovim-boroshnom/viewer
Paska, M., Markovych, I. (2015). Use of plant raw materials in the production technologies smoked sausages. State and prospects of food science and industry, 82–83. Available at: https://elartu.tntu.edu.ua/bitstream/123456789/6458/2/FSI_2015_Paska_M-Use_of_plant_raw_materials_82-83.pdf
Bernyk, I. M., Novgorodska, N. V., Ovsienko, S. M. (2024). Technology of the boiled-smoked sausage products is for the use of side foods of processing of oil-bearing production. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Food Technologies, 26 (101), 26–34. https://doi.org/10.32718/nvlvet-f10105
Kalyna, V. S., Lutsenko, M. V. (2022). Study of the properties of pumpkin seed processing products. Science, Technologies, Innovation, 1 (21), 22–28. https://doi.org/10.35668/2520-6524-2022-1-04
Tischenko, V., Bozhko, N., Pasichnyi, V., Bozhko, S., Marynin, A. (2024). Study of oxidative processes of semi-cooked sausages in modified casings. Scientific Works of National University of Food Technologies, 30 (1), 161–170. https://doi.org/10.24263/2225-2924-2024-30-1-16
Tayeva, A., Kozhakhiyeva, M., Jetpisbayeva, B., Tlevlessova, D., Samadun, A., Valiyv, A. (2023). Development of technology of boiled sausage from non-traditional raw materials. Eastern-European Journal of Enterprise Technologies, 2 (11 (122)), 15–23. https://doi.org/10.15587/1729-4061.2023.277494
Elavarasan, K., Malini, M., Ninan, G., Ravishankar, C. N., Dayakar, B. R. (2024). Millet flour as a potential ingredient in fish sausage for health and sustainability. Sustainable Food Technology, 2 (4), 1088–1100. https://doi.org/10.1039/d4fb00067f
James, R. M., Krishnamoorthy, E. (2025). Coconut Flour as a Novel Functional Ingredient in Tilapia Fish Sausage: Effect on Textural and Sensory Properties. Food Safety and Health. https://doi.org/10.1002/fsh3.70004
Umesha Bhatta, B., Prabhu, R. M., Manjunatha Reddy, A., Elavarasan, K. (2015). Biochemical Changes in DressedPriacanthus hamrur(Bull's Eye) During Frozen Storage and Its Effect on Physical and Sensory Quality of Fish Sausage. International Journal of Food Properties, 18 (4), 897–908. https://doi.org/10.1080/10942912.2013.837062
You, S., Yang, S., Li, L., Zheng, B., Zhang, Y., Zeng, H. (2022). Processing Technology and Quality Change during Storage of Fish Sausages with Textured Soy Protein. Foods, 11 (22), 3546. https://doi.org/10.3390/foods11223546
Gómez-Guillén, M. C., Giménez, B., López-Caballero, M. E., Montero, M. P. (2011). Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloids, 25 (8), 1813–1827. https://doi.org/10.1016/j.foodhyd.2011.02.007
Ranasinghe, R. A. S. N., Wijesekara, W. L. I., Perera, P. R. D., Senanayake, S. A., Pathmalal, M. M., Marapana, R. A. U. J. (2020). Functional and Bioactive Properties of Gelatin Extracted from Aquatic Bioresources – A Review. Food Reviews International, 38 (4), 812–855. https://doi.org/10.1080/87559129.2020.1747486
Zang, E., Jiang, L., Cui, H., Li, X., Yan, Y., Liu, Q. et al. (2022). Only Plant-based Food Additives: An Overview on Application, Safety, and Key Challenges in the Food Industry. Food Reviews International, 39 (8), 5132–5163. https://doi.org/10.1080/87559129.2022.2062764
Markovych, I. I. (2015). Investigation of fatty acid smoked sausages with use lentils, juniper and thyme. Journal of Food Science and Technology, 9 (1). https://doi.org/10.15673/2073-8684.30/2015.38383
Novais, C., Molina, A. K., Abreu, R. M. V., Santo-Buelga, C., Ferreira, I. C. F. R., Pereira, C. et al. (2022). Natural Food Colorants and Preservatives: A Review, a Demand, and a Challenge. Journal of Agricultural and Food Chemistry, 70 (9), 2789–2805. https://doi.org/10.1021/acs.jafc.1c07533
Pasichnyi, V., Shevchenko, O., Tischenko, V., Bozhko, N., Marynin, A., Strashynskyi, I., Matsuk, Y. (2024). Substantiating the feasibility of using hemp seed protein in cooked sausage technology. Eastern-European Journal of Enterprise Technologies, 4 (11 (130)), 56–66. https://doi.org/10.15587/1729-4061.2024.310668
Leonov, O. A., Shkaruba, N. Z. (2021). Quality and safety monitoring production of boiled-smoked sausages. IOP Conference Series: Earth and Environmental Science, 677 (2), 022089. https://doi.org/10.1088/1755-1315/677/2/022089
Ren, Y., Huang, L., Zhang, Y., Li, H., Zhao, D., Cao, J., Liu, X. (2022). Application of Emulsion Gels as Fat Substitutes in Meat Products. Foods, 11 (13), 1950. https://doi.org/10.3390/foods11131950
Buzhanska, M. V., Oshchypok, I. M. (2022). Physico-chemical properties of starch and starch products as an advantage of their use in food industry. Scientific Works of National University of Food Technologies, 28 (1), 145–153. https://doi.org/10.24263/2225-2924-2022-28-1-14
Domínguez, R., Pateiro, M., Gagaoua, M., Barba, F. J., Zhang, W., Lorenzo, J. M. (2019). A Comprehensive Review on Lipid Oxidation in Meat and Meat Products. Antioxidants, 8 (10), 429. https://doi.org/10.3390/antiox8100429
Ashakayeva, R., Assenova, B., Tumenova, G., Nurgazezova, A., Zhumanova, G., Atambayeva, Z. et al. (2022). A Pumpkin-Based Emulsion Gel as a Texture Improvement of Mixed Horsemeat Semi-Smoked Sausages. Foods, 11 (23), 3886. https://doi.org/10.3390/foods11233886
Shahidi, F., Zhong, Y. (2010). Lipid oxidation and improving the oxidative stability. Chemical Society Reviews, 39 (11), 4067–4079. https://doi.org/10.1039/b922183m
Ahmed, I., Jan, K., Fatma, S., Dawood, M. A. O. (2022). Muscle proximate composition of various food fish species and their nutritional significance: A review. Journal of Animal Physiology and Animal Nutrition, 106 (3), 690–719. Portico. https://doi.org/10.1111/jpn.13711
Valentim, J., Afonso, C., Gomes, R., Gomes-Bispo, A., Prates, J. A. M., Bandarra, N. M., Cardoso, C. (2024). Influence of cooking methods and storage time on colour, texture, and fatty acid profile of a novel fish burger for the prevention of cognitive decline. Heliyon, 10 (5), e27171. https://doi.org/10.1016/j.heliyon.2024.e27171
Statkevych, O. I., Kolomiiets, Y. V., Holembovska, N. V., Israelian, V. M., Babych, O. A., Slobodyanyuk, N. M. et al. (2024). Effects of nutrient medium on various-age larvae of Hermetia illucens (Diptera, Stratiomyidae). Regulatory Mechanisms in Biosystems, 15 (4), 907–911. https://doi.org/10.15421/0224131
Bayram, I., Decker, E. A. (2023). Underlying mechanisms of synergistic antioxidant interactions during lipid oxidation. Trends in Food Science & Technology, 133, 219–230. https://doi.org/10.1016/j.tifs.2023.02.003
Feng, L., Tang, N., Liu, R., Gong, M., Wang, Z., Guo, Y. et al. (2021). The relationship between flavor formation, lipid metabolism, and microorganisms in fermented fish products. Food & Function, 12 (13), 5685–5702. https://doi.org/10.1039/d1fo00692d
DSTU ISO 5508-2001. Animal and vegetable fats and oils. Analysis of fatty acid methyl esters by gas chromatography. General specifications. Quality management systems – Requirements.
STU ISO 5509-2002 Animal and vegetable fats and oils. Preparation of fatty acid methyl esters. General specifications. Quality management systems – Requirements.

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.